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Image Search Results
Journal: bioRxiv
Article Title: The C-terminal domain of Hsp70 is responsible for paralog-specific regulation of ribonucleotide reductase
doi: 10.1101/2022.02.08.479504
Figure Lengend Snippet: (A-C) Cells expressing either Ssa1, 2, 3 or 4 as their sole Ssa and HA-tagged Rnr1/Rnr2/Rnr4 were grown to exponential phase and were either left untreated or were treated with 200 mM HU for 3 hrs. HA-RNR complexes were immunoprecipitated with anti-HA magnetic beads and were subjected to SDS-PAGE and analyzed by immunoblotting with anti-HA antibodies to detect the RNR subunits or anti-Ydj1 antibodies to detect Ydj1. (D) Interaction between RNR and Ydj1 (Ydj1/RNR) was calculated by quantitating bands from three replicate experiment. Each value represents the mean ± SD (n = 3). Statistical significance between samples was calculated ANOVA. (∗∗∗∗ p < 0.01).
Article Snippet: Proteins were detected using the following antibodies; anti-HA tag (Thermo #26183), Anti-FLAG tag (Sigma, #F1365), anti-PGK1 (Thermo # PA5-28612),
Techniques: Expressing, Immunoprecipitation, Magnetic Beads, SDS Page, Western Blot
Journal: Cell Metabolism
Article Title: Estradiol regulates leptin sensitivity to control feeding via hypothalamic Cited1
doi: 10.1016/j.cmet.2023.02.004
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Plasmid Preparation, Recombinant, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Diagnostic Assay, Expressing, Software